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Product Info Summary
| SKU: | M01253 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cortactin CTTN Rabbit Monoclonal Antibody
SKU/Catalog Number
M01253
BM4485 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cortactin CTTN Rabbit Monoclonal Antibody catalog # M01253. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cortactin CTTN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01253)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FAI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cortactin
Reactive Species
M01253 is reactive to CTTN in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
61.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01253 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human A431 whole cell, human Caco-2 whole cell, rat brain tissue, mouse brain tissue
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cortactin using anti-Cortactin antibody (M01253).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cortactin antigen affinity purified monoclonal antibody (Catalog # M01253) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cortactin at approximately 85 kDa. The expected band size for Cortactin is at 62 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin Antibody.
Specific Publications For Anti-Cortactin CTTN Rabbit Monoclonal Antibody (M01253)
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Customer Q&As
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5 Customer Q&As for Anti-Cortactin CTTN Rabbit Monoclonal Antibody
Question
We have observed staining in human mammary gland. What should we do? Is anti-Cortactin Rabbit Monoclonal antibody supposed to stain mammary gland positively?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
From literature mammary gland does express CTTN. From Uniprot.org, CTTN is expressed in right ovary, mammary gland, placenta testis, leukemic t-cell, cervix carcinoma, prostate cancer, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CTTN expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 1532244, 8474448
Placenta, and Testis, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Prostate cancer, Pubmed ID: 17487921
Boster Scientific Support
Answered: 2020-04-27
Question
My colleagues were satisfied with the WB result of your anti-Cortactin Rabbit Monoclonal antibody. However we have seen positive staining in mammary gland cytoskeleton using this antibody. Is that expected? Could you tell me where is CTTN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
According to literature, mammary gland does express CTTN. Generally CTTN expresses in cytoplasm, cytoskeleton. Regarding which tissues have CTTN expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 1532244, 8474448
Placenta, and Testis, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Prostate cancer, Pubmed ID: 17487921
Boster Scientific Support
Answered: 2020-04-07
Question
I am interested in using your anti-Cortactin Rabbit Monoclonal antibody for membrane organization studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
N. Dhar
Verified customer
Asked: 2019-08-23
Answer
We appreciate your inquiry. This M01253 anti-Cortactin Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-23
Question
We ordered your anti-Cortactin Rabbit Monoclonal antibody for IHC-P on right ovary in a previous experiment. I am using human, and We are going to use the antibody for WB next. I am looking for examining right ovary as well as prostate cancer in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I have checked the website and datasheets of our anti-Cortactin Rabbit Monoclonal antibody and it seems that M01253 has been tested on human in both IHC-P and WB. Thus M01253 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-Cortactin Rabbit Monoclonal antibody M01253 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
K. Taylor
Verified customer
Asked: 2017-05-30
Answer
The anti-Cortactin Rabbit Monoclonal antibody (M01253) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-30
