Anti-Cortactin CTTN Rabbit Monoclonal Antibody

Boster Bio Anti-Cortactin CTTN Rabbit Monoclonal Antibody catalog # M01253. Tested in Flow Cytometry, IP, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01253
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC-P, ICC, WB

Product Name

Anti-Cortactin CTTN Rabbit Monoclonal Antibody

See all Cortactin products

SKU/Catalog Number

M01253

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cortactin CTTN Rabbit Monoclonal Antibody catalog # M01253. Tested in Flow Cytometry, IP, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cortactin CTTN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01253)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FAI-3

Immunogen

A synthesized peptide derived from human Cortactin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01253 is reactive to CTTN in Human, Mouse, Rat

Applications

M01253 is guaranteed for Flow Cytometry, IP, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC-P 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Flow Cytometry 1:20

Validation Images & Assay Conditions

Gene/Protein Information For CTTN (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14247

Gene ID

2017

Gene Name

CTTN

Full Name

Src substrate cortactin

Weight

61.586kDa

Alternative Names

1110020L01Rik; Amplaxin; Cortactin; CTTN; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate); EMS1; EMS1amplaxin; FLJ34459; Oncogene EMS1; src substrate cortactin CTTN EMS1 cortactin src substrate cortactin|1110020L01Rik|amplaxin|ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)|epididymis secretory sperm binding protein|oncogene EMS1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTTN, check out the CTTN Infographic

CTTN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01253

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5 Customer Q&As for Anti-Cortactin CTTN Rabbit Monoclonal Antibody

Question

We have observed staining in human mammary gland. What should we do? Is anti-Cortactin Rabbit Monoclonal antibody supposed to stain mammary gland positively?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

From literature mammary gland does express CTTN. From Uniprot.org, CTTN is expressed in right ovary, mammary gland, placenta testis, leukemic t-cell, cervix carcinoma, prostate cancer, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CTTN expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 1532244, 8474448
Placenta, and Testis, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Prostate cancer, Pubmed ID: 17487921

Boster Scientific Support

Answered: 2020-04-27

Question

My colleagues were satisfied with the WB result of your anti-Cortactin Rabbit Monoclonal antibody. However we have seen positive staining in mammary gland cytoskeleton using this antibody. Is that expected? Could you tell me where is CTTN supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

According to literature, mammary gland does express CTTN. Generally CTTN expresses in cytoplasm, cytoskeleton. Regarding which tissues have CTTN expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 1532244, 8474448
Placenta, and Testis, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Prostate cancer, Pubmed ID: 17487921

Boster Scientific Support

Answered: 2020-04-07

Question

I am interested in using your anti-Cortactin Rabbit Monoclonal antibody for membrane organization studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

N. Dhar

Verified customer

Asked: 2019-08-23

Answer

We appreciate your inquiry. This M01253 anti-Cortactin Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-23

Question

We ordered your anti-Cortactin Rabbit Monoclonal antibody for IHC-P on right ovary in a previous experiment. I am using human, and We are going to use the antibody for WB next. I am looking for examining right ovary as well as prostate cancer in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

I have checked the website and datasheets of our anti-Cortactin Rabbit Monoclonal antibody and it seems that M01253 has been tested on human in both IHC-P and WB. Thus M01253 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-09

Question

We are currently using anti-Cortactin Rabbit Monoclonal antibody M01253 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

K. Taylor

Verified customer

Asked: 2017-05-30

Answer

The anti-Cortactin Rabbit Monoclonal antibody (M01253) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-30

Size

Total: $315

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M01253
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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