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Product Info Summary
| SKU: | A15662-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-COX6A2 Antibody (Center)
SKU/Catalog Number
A15662-2
Size
80 µl
Description
Boster Bio Anti-COX6A2 Antibody (Center) (Catalog # A15662-2). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-COX6A2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15662-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This COX6A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human COX6A2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15662-2 is reactive to COX6A2 in Human
Applications
A15662-2 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
10.815kDa
Background of COX6A2
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from human skeletal muscle tissue lysate, using COX6A2 Antibody (Center). A15662-2 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of lysate from human heart tissue lysate, using COX6A2 Antibody (Center). A15662-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using COX6A2 Antibody (Center). A15662-2 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For COX6A2 (Source: Uniprot.org, NCBI)
Gene Name
COX6A2
Full Name
Cytochrome c oxidase subunit 6A2, mitochondrial
Weight
10.815kDa
Superfamily
cytochrome c oxidase subunit 6A family
Alternative Names
COX VIa-M; COX6A; COX6AH; COXVIAH; Cytochrome c oxidase polypeptide VIa-heart; cytochrome c oxidase subunit 6A2, mitochondrial; cytochrome c oxidase subunit VIa polypeptide 2; Cytochrome c oxidase subunit VIA-muscle COX6A2 COX6AH, COXVIAH, MC4DN18 cytochrome c oxidase subunit 6A2 cytochrome c oxidase subunit 6A2, mitochondrial|COX VIa-M|cytochrome c oxidase polypeptide VIa-heart|cytochrome c oxidase subunit VIA-muscle|cytochrome c oxidase subunit VIa polypeptide 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COX6A2, check out the COX6A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COX6A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COX6A2 Antibody (Center) (A15662-2)
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