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Product Info Summary
| SKU: | A15915 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-COXFA4L2 Antibody Picoband®
SKU/Catalog Number
A15915
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-COXFA4L2 Antibody Picoband® catalog # A15915. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-COXFA4L2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15915)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human COXFA4L2.(ASLGARFYRQIKRHPGIIPMIAC)
Reactive Species
A15915 is reactive to COXFA4L2 in Human
Observed Molecular Weight
14 kDa
Calculated molecular weight
10.0 kDa
Background of COXFA4L2
Located in mitochondrion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15915 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human |
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, COLO cells, HUVEC cells and HepG2 cells, using NDUFA4L2 antibody A15915.
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Western blot analysis of COXFA4L2 using anti-COXFA4L2 antibody (A15915).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COXFA4L2 antigen affinity purified polyclonal antibody (A15915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COXFA4L2 at approximately 14 kDa. The expected band size for COXFA4L2 is at 10 kDa.
Specific Publications For Anti-COXFA4L2 Antibody Picoband® (A15915)
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