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Product Info Summary
| SKU: | A30602 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CRABP2 Antibody
SKU/Catalog Number
A30602
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CRABP2 Antibody (Catalog# A30602). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRABP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30602)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CRABP2. Immunogen sequence location: 41-90
Reactive Species
A30602 is reactive to CRABP2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
15.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30602 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using CRABP-II Polyclonal Antibody
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Western blot analysis of lysates from HT-29 cells, using CRABP2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CRABP2 Antibody (A30602)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CRABP2 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ectocervix using anti-CRABP2 antibody A30602. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
We are interested in to test anti-CRABP2 antibody A30602 on human ectocervix for research purposes, then I may be interested in using anti-CRABP2 antibody A30602 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The products we sell, including anti-CRABP2 antibody A30602, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-15
Question
I see that the anti-CRABP2 antibody A30602 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-CRABP2 antibody A30602 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
The anti-CRABP2 antibody (A30602) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-15
Question
Would anti-CRABP2 antibody A30602 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Our lab technicians have not tested anti-CRABP2 antibody A30602 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CRABP2 antibody A30602 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-03
