Click image to see more details
Product Info Summary
| SKU: | M05421 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-CRALBP RLBP1 Monoclonal Antibody
SKU/Catalog Number
M05421
BM5584 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CRALBP RLBP1 Monoclonal Antibody catalog # M05421. Tested in WB, IP applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRALBP RLBP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05421)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEFB-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CRALBP Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors.
Reactive Species
M05421 is reactive to RLBP1 in Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05421 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: mouse eyeball
Validation Images & Assay Conditions
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Western blot analysis of CRALBP expression in mouse eyeball lysate.
Specific Publications For Anti-CRALBP RLBP1 Monoclonal Antibody (M05421)
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Customer Q&As
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7 Customer Q&As for Anti-CRALBP RLBP1 Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-CRALBP Monoclonal antibody M05421. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use your anti-CRALBP Monoclonal antibody for IP for rat uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat uterus identification?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can see on the product datasheet, M05421 anti-CRALBP Monoclonal antibody has been tested for IP, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
I have a question about product M05421, anti-CRALBP Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05421 anti-CRALBP Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-25
Question
Will anti-CRALBP Monoclonal antibody M05421 work on canine WB with uterus?
K. Jackson
Verified customer
Asked: 2018-12-17
Answer
Our lab technicians have not validated anti-CRALBP Monoclonal antibody M05421 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CRALBP Monoclonal antibody M05421 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-17
Question
Here is the WB image, lot number and protocol we used for uterus using anti-CRALBP Monoclonal antibody M05421. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-03
Question
Will anti-CRALBP Monoclonal antibody M05421 work for IP with uterus?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
According to the expression profile of uterus, RLBP1 is highly expressed in uterus. So, it is likely that anti-CRALBP Monoclonal antibody M05421 will work for IP with uterus.
Boster Scientific Support
Answered: 2017-11-09
Question
We are currently using anti-CRALBP Monoclonal antibody M05421 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
The anti-CRALBP Monoclonal antibody (M05421) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-08
