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Product Info Summary
| SKU: | M04247 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CRCP Rabbit Monoclonal Antibody
SKU/Catalog Number
M04247
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CRCP Rabbit Monoclonal Antibody catalog # M04247. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRCP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04247)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
30C57
Isotype
IgG
Immunogen
A synthesized peptide derived from human CRCP
Reactive Species
M04247 is reactive to CRCP in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
16.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04247 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of CRCP expression in HeLa cell lysate.
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Western blot analysis of CRCP using anti-CRCP antibody (M04247).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: 2h drug treated-human MCF-7 whole cell lysates,
Lane 3: 4h drug treated-human MCF-7 whole cell lysates,
Lane 4: 12h drug treated-human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRCP antigen affinity purified monoclonal antibody (Catalog # M04247) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for CRCP at approximately 17kDa. The expected band size for CRCP is at 16.9 kDa.
Specific Publications For Anti-CRCP Rabbit Monoclonal Antibody (M04247)
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Customer Reviews
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1 Reviews For Anti-CRCP Rabbit Monoclonal Antibody
This antibody is highly specific and efficient, suitable for Western blot detection of CRCP protein in MCF-7 cells, with only minor nonspecific bands observed.
Excellent
| SKU | M04247 |
|---|---|
| Application | Western Blot |
| Sample | Human mcf-7 cells |
| Sample Processing Description | RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish, and the cells were lysed on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted to equal concentrations. Subsequently, 5× protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE electrophoresis. |
| Other Reagents | Blocking buffer |
| Primary Antibody | CRCP Rabbit Monoclonal Antibody |
| Primary Incubation | 1:500, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | As shown in the figure, the expression levels of CRCP protein in MCF-7 cells at different drug treatment time points are presented. The Western blot results obtained with this antibody are clear; although there are a few faint nonspecific bands above, they do not affect result interpretation. It can be observed that the expression of the target protein in MCF-7 cells increases with longer drug treatment times. |
Siyuan Bu, LUTCM
Verified customer
Submitted 2025-12-26
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