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Product Info Summary
| SKU: | M01695 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Creatine kinase B type CKB Monoclonal Antibody
SKU/Catalog Number
M01695
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Creatine kinase B type CKB Monoclonal Antibody catalog # M01695. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Creatine kinase B type CKB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01695)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFOC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Creatine kinase B type Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01695 is reactive to CKB in Human, Mouse, Rat
Applications
M01695 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
v
Calculated molecular weight
42.644kDa
Background of CKB
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of Creatine kinase B type expression in SHSY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CKB (Source: Uniprot.org, NCBI)
Gene Name
CKB
Full Name
Creatine kinase B-type
Weight
42.644kDa
Superfamily
ATP:guanido phosphotransferase family
Alternative Names
B-CK; CKB; Creatine Kinase BB CKB B-CK, BCKB, CPK-B, HEL-211, HEL-S-29, CKB creatine kinase B creatine kinase B-type|brain creatine kinase|creatine kinase B chain|creatine kinase brain|creatine kinase brain-type|creatine phosphokinase B-type|epididymis luminal protein 211|epididymis secretory protein Li 29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CKB, check out the CKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Creatine kinase B type CKB Monoclonal Antibody (M01695)
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No publications found for M01695
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Creatine kinase B type CKB Monoclonal Antibody
Question
I was wanting to use your anti-Creatine kinase B type Monoclonal antibody for IP for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
You can see on the product datasheet, M01695 anti-Creatine kinase B type Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
Does anti-Creatine kinase B type Monoclonal antibody M01695 work for IP with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CKB is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Creatine kinase B type Monoclonal antibody M01695 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-05-30
Question
Do you have a BSA free version of anti-Creatine kinase B type Monoclonal antibody M01695 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Creatine kinase B type Monoclonal antibody M01695 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Does M01695 anti-Creatine kinase B type Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Banerjee
Verified customer
Asked: 2018-07-27
Answer
You can see on the product datasheet, M01695 anti-Creatine kinase B type Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-27
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Creatine kinase B type Monoclonal antibody M01695. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-24
Question
My question regarding product M01695, anti-Creatine kinase B type Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Banerjee
Verified customer
Asked: 2015-05-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01695 anti-Creatine kinase B type Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-05-20
