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Product Info Summary
| SKU: | A10681 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CRISP2 Antibody
SKU/Catalog Number
A10681
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CRISP2 Antibody (Catalog # A10681). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
CRISP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CRISP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10681)
Host
Rabbit
Contents
CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
CRISP2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2. The immunogen is located within the first 50 amino acids of CRISP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of MTERFD2 are known to exist. MTERFD2 antibody is predicted to not cross-react with other MTERFD protein family members.
Reactive Species
A10681 is reactive to CRISP2 in Human
Applications
A10681 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.259kDa
Background of CRISP-2
The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of CRISP2 in human testis tissue with CRISP2 antibody at 10 μg/mL.
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Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CRISP2 (Source: Uniprot.org, NCBI)
Gene Name
CRISP2
Full Name
Cysteine-rich secretory protein 2
Weight
27.259kDa
Superfamily
CRISP family
Alternative Names
Cancer/testis antigen 36; CRISP2; CRISP-2; CRISP-2GAPDL5; CT36TSP1; cysteine-rich secretory protein 2; GAPDL5; glyceraldehyde-3-phosphate dehydrogenase-like 5; testis specific protein 1 (probe H4-1 p3-1); Testis-specific protein TPX-1; TPX1; TPX1MGC111136; TSP1 CRISP2 CRISP-2, CT36, GAPDL5, TPX1, TSP1 cysteine rich secretory protein 2 cysteine-rich secretory protein 2|cancer/testis antigen 36|glyceraldehyde-3-phosphate dehydrogenase-like 5|testicular tissue protein Li 43|testis specific protein 1 (probe H4-1 p3-1)|testis-specific protein TPX-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRISP2, check out the CRISP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRISP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRISP2 Antibody (A10681)
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