Anti-CRISPLD2 Antibody

Product Name

Anti-CRISPLD2 Antibody

View all Crispld2 Antibodies

SKU/Catalog Number

A07439

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CRISPLD2 Antibody catalog # A07439. Tested in WB applications. This antibody reacts with Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CRISPLD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07439)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NEB2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07439 is reactive to Crispld2 in Mouse, Rat

Applications

A07439 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.432kDa

Background of Crispld2

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

Izaurralde E., Cell 78:657-668(1994).
Kataoka N., Nucleic Acids Res. 22:3861-3865(1994).
Humphray S.J., Nature 429:369-374(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-CRISPLD2 Antibody A07439

Western blot analysis of extracts of various cell lines

Gene/Protein Information For Crispld2 (Source: Uniprot.org, NCBI)

Alternative Names

CRISP11; CRISP-11; Cysteine-rich secretory protein 11; cysteine-rich secretory protein LCCL domain containing 2; cysteine-rich secretory protein LCCL domain-containing 2; DKFZP434B044; LCCL domain containing cysteine-rich secretory protein 2; LCCL domain-containing cysteine-rich secretory protein 2; LCRISP2; MGC74865 Crispld2|1810049K24Rik, Lcrisp2, Lg, Lgl1, coffee, coffeecrisp|cysteine-rich secretory protein LCCL domain containing 2|cysteine-rich secretory protein LCCL domain-containing 2

For more info on Crispld2, check out the Crispld2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Crispld2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Crispld2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis