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- Table of Contents
cas9 antibody (M30929) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Recombinant protein.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody .
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Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
cas9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Recombinant protein | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
cas9 antibody was positively detected by WB in:
293T cell, 3T3 cell, PC12 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
cas9 antibody was positively detected by ICC/IF in:
293T cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
FC
Human
Recommend
Recommend
Recombinant protein
Recommend
Recommend
Product Information
Product Name
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
View all CAS9 Antibodies
View all CAS9 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABCI-3
Isotype
Rabbit IgG
Immunogen
Recombinant fragment derived from Streptococcus pyogenes.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
158 kDa
Cite This Product
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30929)
Protocols
Western Blot protocol for cas9 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for cas9 antibody
Publications
Application:
Species:
Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic loci
Published: 2025-08-28
Journal: Cell Genomics
Application: WB
Impact Factor: 9.00
Species: Human
PMID: 40882622
Decreasing predictable DNA off-target effects and narrowing editing windows of adenine base editors by fusing human Rad18 protein variant
Published: 2023-10-16
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 37848112
Review
Have you used Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody?
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1 Review For Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.
| SKU | M30929 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | Anti-Rabbit IgG Secondary Antibody, HRP-conjugated |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Azure Biosystems c600. |
| Results Summary | I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues. |
Ziru Wang, Jilin University
Verified customer
Submitted 2025-10-10
Product Promise
We promise Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M30929
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026