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Product Info Summary
| SKU: | M30929 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Recombinant protein |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
SKU/Catalog Number
M30929
BM5120 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody catalog # M30929. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant protein.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30929)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABCI-3
Isotype
Rabbit IgG
Immunogen
Recombinant fragment derived from Streptococcus pyogenes.
Reactive Species
M30929 is reactive to cas9 in Human, Recombinant protein
Observed Molecular Weight
158 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30929 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: 293T cell, 3T3 cell, PC12 cell
ICC/IF: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
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Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody .
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Western blot analysis of CRISPR-Cas9 SP using anti-CRISPR-Cas9 SP antibody (A04887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-3: 24h human HEK293T whole cell lysates,
Lane 4-6: 48h human HEK293T whole cell lysates,
Lane 7-9: 60h human HEK293T whole cell lysates,
Lane 10-12: 72h human A549 whole cell lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRISPR-Cas9 SP antigen affinity purified polyclonal antibody (A04887-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Azure Biosystems c600 system. A specific band was detected for CRISPR-Cas9 SP at approximately 158 kDa. The expected band size for CRISPR-Cas9 SP is at 158 kDa.
Specific Publications For Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody (M30929)
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Customer Reviews
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1 Reviews For Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.
Excellent
| SKU | M30929 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | Anti-Rabbit IgG Secondary Antibody, HRP-conjugated |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Signal was developed using ECL substrate on a Azure Biosystems c600. |
| Results Summary | I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues. |
Ziru Wang, Jilin University
Verified customer
Submitted 2025-10-10
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