Anti-CRMP4/Dpysl3 Antibody Picoband™

Product Name

Anti-CRMP4/Dpysl3 Antibody Picoband™

View all CRMP4 Antibodies

SKU/Catalog Number

A05960-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CRMP4/Dpysl3 Antibody Picoband™ catalog # A05960-1. Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CRMP4/Dpysl3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05960-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat CRMP4/Dpysl3, which shares 93.3% amino acid (aa) sequence identity with both human and mouse CRMP4/Dpysl3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05960-1 is reactive to DPYSL3 in Rat

Applications

A05960-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

62 kDa

Calculated molecular weight

61.963kDa

Background of CRMP4

Dihydropyrimidinase-related protein 3 is an enzyme that in humans is encoded by the DPYSL3 gene. This gene encodes a protein that belongs to the TUC (TOAD-64/Ulip/CRMP) family of proteins. Members of this family are phosphoproteins that function in axonal guidance and neuronal differentiation during development and regeneration of the nervous system. A mutation in the human gene is associated with amyotrophic lateral sclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CRMP4/Dpysl3 using anti-CRMP4/Dpysl3 antibody (A05960-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRMP4/Dpysl3 antigen affinity purified polyclonal antibody (Catalog # A05960-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRMP4/Dpysl3 at approximately 62 kDa. The expected band size for CRMP4/Dpysl3 is at 62 kDa.

Gene/Protein Information For DPYSL3 (Source: Uniprot.org, NCBI)

Alternative Names

Collapsin response mediator protein 4; CRMP-4; CRMP4collapsin response mediator protein 4 long; dihydropyrimidinase-like 3; DRP-3dihydropyrimidinase-related protein 3; DRP3ULIP1; ULIP-1; ULIPLCRMP; Unc-33-like phosphoprotein 1 DPYSL3 CRMP-4, CRMP4, DRP-3, DRP3, LCRMP, ULIP, ULIP-1 dihydropyrimidinase like 3 dihydropyrimidinase-related protein 3|collapsin response mediator protein 4 long|testicular secretory protein Li 7|unc-33-like phosphoprotein 1

For more info on DPYSL3, check out the DPYSL3 Infographic

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In this infographic, you will see the following information for DPYSL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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