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Product Info Summary
| SKU: | A05114 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-CSDE1 Antibody
SKU/Catalog Number
A05114
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CSDE1 Antibody catalog # A05114. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSDE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05114)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human CSDE1(NP_009089.4).
Reactive Species
A05114 is reactive to CSDE1 in Human, Mouse
Observed Molecular Weight
111 kDa
Calculated molecular weight
88.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05114 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:20-1:50
Validation Images & Assay Conditions
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Western blot analysis of extracts from normal and CSDE1 knockout HeLa cells, using CSDE1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.
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Immunofluorescence analysis of L929 cells using CSDE1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug CSDE1 antibody . Western blot was performed from the immunoprecipitate using CSDE1 antibody at a dilution of 1:1000.
Specific Publications For Anti-CSDE1 Antibody (A05114)
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