This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CSF1 antibody (A00620-3) performs well in ELISA, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
Western blot analysis of CSF1 using anti-CSF1 antibody (A00620-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human 293T whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 antigen affinity purified polyclonal antibody (Catalog # A00620-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1 at approximately 60KD. The expected band size for CSF1 is at 60KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human 293T whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 antigen affinity purified polyclonal antibody (Catalog # A00620-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1 at approximately 60KD. The expected band size for CSF1 is at 60KD.
Bundle Save
Anti-CSF1 Antibody Picoband®
CSF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, WB |
Tested Applications
CSF1 antibody was positively detected by WB in:
human placenta tissue, human 293T whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
ELISA
Human
Tested
Recommend
Mouse
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-CSF1 Antibody Picoband®
View all CSF1 Antibodies
View all CSF1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CSF1 recombinant protein (Position: E33-N190).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
60.2 kDa
Background of CSF1
M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Cite This Product
Anti-CSF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00620-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
90%
High Homology
Cow
89%
Moderate Homology
Sheep
89%
Moderate Homology
Rabbit
84%
Moderate Homology
Mouse
82%
Moderate Homology
Rat
75%
Moderate Homology
Zebrafish
25%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CSF1 antibody
Publications
Application:
Species:
Genetically engineered bacteria expressing IL-34 alleviate DSS-induced experimental colitis by promoting tight junction protein expression in intestinal mucosal epithelial cells
Published: 2025-01-25
Journal: MOLECULAR IMMUNOLOGY
Application: WB
Impact Factor: 3.00
Species: Human
PMID: 39864284
Epigenetic silencing of miR-130b in ovarian cancer promotes the development of multidrug resistance by targeting colony-stimulating factor 1
Published: 2011-10-15
Journal: GYNECOLOGIC ONCOLOGY
Impact Factor: 3.76
Species: Human
PMID: 22005523
Review
Have you used Anti-CSF1 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-CSF1 Antibody Picoband®
Anti-CSF1 Antibody (A00620-3) produced distinct target bands in WB assays using mouse macrophages, with only faint non-specific bands observed and consistent GAPDH loading control.
| SKU | A00620-3 |
|---|---|
| Application | Western Blot |
| Sample | Mouse macrophages |
| Sample Processing Description | Cells were lysed in RIPA lysis buffer supplemented with PMSF (100:1) for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected and mixed with 5× Loading Buffer. Samples were denatured at 100°C for 10 min and then loaded onto an SDS-PAGE gel. |
| Other Reagents | Blocking buffer: 5% non-fat milk |
| Primary Antibody | CSF1 Antibody Picoband® |
| Primary Incubation | 1:2,000 dilution, incubated overnight at 4°C. |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody |
| Secondary Incubation | 1:2000, incubated for 1 hour at RT.< |
| Detection | ECL, Imaging system: UNITEC CAMBRIDGE |
| Results Summary | The figure displays representative Western blot results for the target protein CSF1 and the internal control GAPDH in different types of macrophages; the target bands are clear and distinct, and the experimental results are satisfactory. |
Jingting Jia, Capital Medical University
Verified customer
Submitted 2026-09-16
Product Promise
We promise Anti-CSF1 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00620-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026