|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-CSK Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Tyrosine-protein kinase CSK(CSK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-CSK Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9102)|
|Replacement Item||This antibody may replace the following items: sc-9897|sc-13074|sc-166560|sc-51580|sc-286|sc-166513 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tyrosine-protein kinase CSK|
|Molecular Weight||50704 MW|
|Protein Function||Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. .|
|Tissue Specificity||Expressed in lung and macrophages. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.|
|Subcellular Localization||Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. .|
|Alternative Names||Tyrosine-protein kinase CSK;220.127.116.11;C-Src kinase;Protein-tyrosine kinase CYL;CSK;|
|Research Areas|||signal transduction|protein phosphorylation|tyrosine kinases|src family||
Background for Tyrosine-protein kinase CSK
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CSK Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CSK Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CSK (PB9102) at 0.5ug/ml
WB: Recombinant Human CSK Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti CSK (PB9102) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: NIH3T3 Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,