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Product Info Summary
| SKU: | A01485-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Ctip2/BCL11B Antibody Picoband®
SKU/Catalog Number
A01485-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ctip2/BCL11B Antibody Picoband® catalog # A01485-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ctip2/BCL11B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01485-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Ctip2/BCL11B recombinant protein (Position: K838-S894).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01485-1 is reactive to BCL11B in Human, Mouse, Rat
Observed Molecular Weight
130 kDa
Calculated molecular weight
95.5 kDa
Background of BCL11B
B-cell lymphoma/leukemia 11B is a protein that in humans is encoded by the BCL11B gene. This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01485-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human MOLT-4 whole cell, rat brain tissue, mouse brain tissue, mouse RAW2647 whole cell, mouse Ana-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Ctip2/BCL11B using anti-Ctip2/BCL11B antibody (A01485-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ctip2/BCL11B antigen affinity purified polyclonal antibody (Catalog # A01485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ctip2/BCL11B at approximately 130 kDa. The expected band size for Ctip2/BCL11B is at 96 kDa.
Specific Publications For Anti-Ctip2/BCL11B Antibody Picoband® (A01485-1)
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