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Product Info Summary
| SKU: | M03447 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03447
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody catalog # M03447. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03447)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
26S95
Isotype
IgG
Immunogen
A synthesized peptide derived from human CTR1/SLC31A1
Reactive Species
M03447 is reactive to SLC31A1 in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
21.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03447 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human K562 whole cell, human CACO-2 whole cell, rat C6 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CTR1/SLC31A1 using anti-CTR1/SLC31A1 antibody (M03447).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTR1/SLC31A1 antigen affinity purified monoclonal antibody (Catalog # M03447) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTR1/SLC31A1 at approximately 29 kDa. The expected band size for CTR1/SLC31A1 is at 21 kDa.
Specific Publications For Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody (M03447)
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