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Product Info Summary
| SKU: | M03447 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody
View all SLC31A1/CTR1 Antibodies
SKU/Catalog Number
M03447
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody catalog # M03447. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03447)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
26S95
Isotype
IgG
Immunogen
A synthesized peptide derived from human CTR1/SLC31A1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03447 is reactive to SLC31A1 in Human, Mouse, Rat
Applications
M03447 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
21.091kDa
Background of SLC31A1/CTR1
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CTR1/SLC31A1 using anti-CTR1/SLC31A1 antibody (M03447).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTR1/SLC31A1 antigen affinity purified monoclonal antibody (Catalog # M03447) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTR1/SLC31A1 at approximately 29 kDa. The expected band size for CTR1/SLC31A1 is at 21 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SLC31A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC31A1
Full Name
High affinity copper uptake protein 1
Weight
21.091kDa
Superfamily
copper transporter (Ctr) (TC 1.A.56) family
Alternative Names
Copper transporter 1; COPT1high affinity copper uptake protein 1; CTR1MGC75487; hCTR1copper transport 1 homolog; solute carrier family 31 (copper transporters), member 1; Solute carrier family 31 member 1 SLC31A1 COPT1, CTR1 solute carrier family 31 member 1 high affinity copper uptake protein 1|copper transport 1 homolog|copper transporter 1|solute carrier family 31 (copper transporter), member 1|solute carrier family 31 (copper transporters), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC31A1, check out the SLC31A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC31A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTR1/SLC31A1 Rabbit Monoclonal Antibody (M03447)
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