Anti-CX3CR1 Antibody Picoband™

Product Name

Anti-CX3CR1 Antibody Picoband™

View all CX3CR1 Antibodies

SKU/Catalog Number

A00280-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CX3CR1 Antibody Picoband™ catalog # A00280-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CX3CR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00280-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CX3CR1 recombinant protein (Position: M1-H345).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00280-2 is reactive to CX3CR1 in Human

Applications

A00280-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

40.396kDa

Background of CX3CR1

CX3C chemokine receptor 1 (CX3CR1) also known as the fractalkine receptor or G-protein coupled receptor 13 (GPR13) is a protein that in humans is encoded by the CX3CR1 gene. Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2).
CX3CR1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 2. IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2).
CX3CR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2).
CX3CR1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of HEL cells using anti-CX3CR1 antibody (A00280-2).
Overlay histogram showing HEL cells stained with A00280-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CX3CR1 Antibody (A00280-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CX3CR1 (Source: Uniprot.org, NCBI)

Alternative Names

Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1 CX3CR1 CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28 C-X3-C motif chemokine receptor 1 CX3C chemokine receptor 1|C-X3-C CKR-1|CMK-BRL-1|CMK-BRL1|G-protein coupled receptor 13|beta chemokine receptor-like 1|chemokine (C-C) receptor-like 1|chemokine (C-X3-C motif) receptor 1|chemokine (C-X3-C) receptor 1|fractalkine receptor

For more info on CX3CR1, check out the CX3CR1 Infographic

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In this infographic, you will see the following information for CX3CR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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