Click image to see more details
Product Info Summary
| SKU: | PB9858 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CXCL2 Antibody Picoband®
SKU/Catalog Number
PB9858
PB0900 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCL2 Antibody Picoband® catalog # PB9858. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9858)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9858 is reactive to Cxcl2 in Mouse, Rat
Observed Molecular Weight
11 kDa
Calculated molecular weight
10.8 kDa
Background of Cxcl2
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9858 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CXCL2 using anti-CXCL2 antibody (PB9858).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat CXCL2 protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL2 antigen affinity purified polyclonal antibody (Catalog # PB9858) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL2 at approximately 11 kDa. The expected band size for CXCL2 is at 11 kDa.
Specific Publications For Anti-CXCL2 Antibody Picoband® (PB9858)
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Customer Q&As
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7 Customer Q&As for Anti-CXCL2 Antibody Picoband®
Question
We are currently using anti-CXCL2 antibody PB9858 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The anti-CXCL2 antibody (PB9858) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-26
Question
Is this PB9858 anti-CXCL2 antibody reactive to the isotypes of CXCL2?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The immunogen of PB9858 anti-CXCL2 antibody is E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-20
Question
I was wanting to use your anti-CXCL2 antibody for ELISA for rat histiocytic lymphoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat histiocytic lymphoma identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
It shows on the product datasheet, PB9858 anti-CXCL2 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat histiocytic lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Does anti-CXCL2 antibody PB9858 work on goat WB with eye?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not validated anti-CXCL2 antibody PB9858 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CXCL2 antibody PB9858 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat eye in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Here is the WB image, lot number and protocol we used for histiocytic lymphoma using anti-CXCL2 antibody PB9858. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Would PB9858 anti-CXCL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
It shows on the product datasheet, PB9858 anti-CXCL2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-CXCL2 antibody PB9858 works with ELISA, what is the protocol used to produce the result images on the product page?
L. Bhatt
Verified customer
Asked: 2018-12-14
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-12-14
