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Product Info Summary
| SKU: | M00295 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-CXCR3 Monoclonal Antibody
SKU/Catalog Number
M00295
BM5285 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CXCR3 Monoclonal Antibody catalog # M00295. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00295)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CXCR3 Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC) . Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC) . May play a role in angiogenesis.
Reactive Species
M00295 is reactive to CXCR3 in Human
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00295 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of CXCR3 expression in K562 cell lysate.
Specific Publications For Anti-CXCR3 Monoclonal Antibody (M00295)
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Customer Q&As
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5 Customer Q&As for Anti-CXCR3 Monoclonal Antibody
Question
What is the immunogen region of the peptide for M00295?
Verified customer
Asked: 2022-03-08
Answer
The immunogen sequence for the Anti-CXCR3 Monoclonal Antibody (M00295) is GALARNCGRESRVDV, aa284-298 (extracellular).
Boster Scientific Support
Answered: 2022-03-09
Question
We have been able to see staining in human brain. Any tips? Is anti-CXCR3 Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
According to literature brain does express CXCR3. According to Uniprot.org, CXCR3 is expressed in leukocyte, blood, endothelial cell thymus, colon, brain, lung testis, fetal astrocyte, among other tissues. Regarding which tissues have CXCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9064356
Brain, Lung, and Testis, Pubmed ID: 15489334
Colon, Pubmed ID: 14702039
Endothelial cell, and Thymus, Pubmed ID: 18291705
Fetal astrocyte, Pubmed ID: 9625760
Boster Scientific Support
Answered: 2018-11-09
Question
We are currently using anti-CXCR3 Monoclonal antibody M00295 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
G. Carter
Verified customer
Asked: 2018-10-18
Answer
The anti-CXCR3 Monoclonal antibody (M00295) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-18
Question
We purchased anti-CXCR3 Monoclonal antibody for WB on blood last year. I am using human, and I plan to use the antibody for IF next. We need examining blood as well as leukocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
I looked at the website and datasheets of our anti-CXCR3 Monoclonal antibody and it appears that M00295 has been validated on human in both WB and IF. Thus M00295 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-31
Question
Our lab were satisfied with the WB result of your anti-CXCR3 Monoclonal antibody. However we have seen positive staining in leukocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CXCR3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
Based on literature, leukocyte does express CXCR3. Generally CXCR3 expresses in isoform 1: cell membrane, isoform 2: cell membrane. Regarding which tissues have CXCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9064356
Brain, Lung, and Testis, Pubmed ID: 15489334
Colon, Pubmed ID: 14702039
Endothelial cell, and Thymus, Pubmed ID: 18291705
Fetal astrocyte, Pubmed ID: 9625760
Boster Scientific Support
Answered: 2017-08-30
