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- Table of Contents
Cxcr3 antibody (A00295-2) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of Cxcr3 using anti-Cxcr3 antibody (A00295-2).
Cxcr3 was detected in a paraffin-embedded section of mouse intestinal lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cxcr3 Antibody (A00295-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cxcr3 was detected in a paraffin-embedded section of mouse intestinal lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cxcr3 Antibody (A00295-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Cxcr3 Antibody Picoband®
Cxcr3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Cxcr3 antibody was positively detected by WB in:
rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cxcr3 antibody was positively detected by IHC in:
mouse intestinal lymph node tissue, rat lymph node tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Cxcr3 Antibody Picoband®
View all CXCR3 Antibodies
View all CXCR3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cxcr3 recombinant protein (Position: M1-E326).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
41.0 kDa
Background of Cxcr3
Chemokine receptor CXCR3 is a Galphai protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. It is mapped to Xq13.1. CXCR3 is expressed on malignant B cells from chronic lymphoproliferative disorders, particularly in patients with CLL, and represents a fully functional receptor involved in chemotaxis of malignant B lymphocytes. It is found that in the absence of known etiologic agents, CXCR3 represents a novel target for therapeutic interference early in type 1 diabetes. CXCR3 signaling is associated with MG pathogenesis and proposed that and CXCR3 may serve as novel drug targets to treat MG. CXCR3A and CXCR3B are involved in the chemotactic and vascular effects of CXCL4L1.
Cite This Product
Anti-Cxcr3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00295-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
96%
High Homology
Human
88%
Moderate Homology
Monkey
88%
Moderate Homology
Rabbit
87%
Moderate Homology
Sheep
86%
Moderate Homology
Cow
86%
Moderate Homology
Dog
85%
Moderate Homology
Zebrafish
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cxcr3 antibody
Immunohistochemistry (IHC) protocol for Cxcr3 antibody
Publications
Application:
Species:
Docosahexaenoic Acid Attenuates Visceral Pain by Suppressing Spinal CXCL10/CXCR3/ERK Signaling
Published: 2026-03-30
Journal: Nutrients
Application: WB,IHC
Impact Factor: 5.00
Species: Mouse
PMID: 41978163
CXCL10 Involvement in Vestibular Migraine via the PI3K/AKT Signaling Pathway
Published: 2025-11-17
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 5.00
Species: Rat
PMID: 41249857
Interior Decorative VOCs Elevate T Cell-Mediated Obstructive Lung Disease Risks via Osteogenesis-Driven Lymphoid-Biased Hematopoiesis
Published: 2025-11-05
Journal: Advanced Science
Application: IF
Impact Factor: 14.10
Species: Mouse
PMID: 41190786
CXCL10/CXCR3 Signaling in the DRG Exacerbates Neuropathic Pain in Mice
Published: 2020-11-16
Journal: Neuroscience Bulletin
Application: IF
Impact Factor: 4.33
Species: Mouse
PMID: 33196963
Chemokine Receptor CXCR3 in the Spinal Cord Contributes to Chronic Itch in Mice
Published: 2017-04-11
Journal: Neuroscience Bulletin
Application: IHC
Impact Factor: 2.62
Species: Mouse
PMID: 28401489
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Product Promise
We promise Anti-Cxcr3 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00295-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026