Anti-Cyclin-T1 CCNT1 Antibody

Product Name

Anti-Cyclin-T1 CCNT1 Antibody

View all CCNT1 Antibodies

SKU/Catalog Number

A02703

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Cyclin-T1 CCNT1 Antibody (Catalog # A02703). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CCNT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Cyclin-T1 CCNT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02703)

Host

Rabbit

Contents

CCNT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rabbit polyclonal CCNT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CCNT1. The immunogen is located within the last 50 amino acids of CCNT1.

Reactive Species

A02703 is reactive to CCNT1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

80.7 kDa

Background of CCNT1

Cyclins function as regulators of CDK kinases and exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin-T1 protein (CCNT1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNT1 tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing CCNT1 and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. CCNT1 and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02703 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CCNT1 antibody can be used for detection of CCNT1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CCNT1

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