Product Info Summary
SKU: | M01308 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody
View all Cyclophilin A Antibodies
SKU/Catalog Number
M01308
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody catalog # M01308. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01308)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AABG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclophilin A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01308 is reactive to PPIA in Human, Mouse, Rat
Applications
M01308 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
51-55 kDa
Calculated molecular weight
18.012kDa
Background of Cyclophilin A
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Cyclophilin A expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PPIA (Source: Uniprot.org, NCBI)
Gene Name
PPIA
Full Name
Peptidyl-prolyl cis-trans isomerase A
Weight
18.012kDa
Superfamily
cyclophilin-type PPIase family
Alternative Names
Cyclophilin A; Cyclosporin A-binding protein; CYPA; CYPAMGC12404; CYPH; EC 5.2.1.8; MGC117158; MGC23397; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase A; Rotamase A; Rotamase; T cell cyclophilin PPIA CYPA, CYPH, HEL-S-69p peptidylprolyl isomerase A peptidyl-prolyl cis-trans isomerase A|PPIase A|T cell cyclophilin|cyclosporin A-binding protein|epididymis secretory sperm binding protein Li 69p|peptidylprolyl isomerase A (cyclophilin A)|rotamase A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPIA, check out the PPIA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody (M01308)
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No publications found for M01308
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-Cyclophilin A Rabbit Monoclonal antibody M01308 available?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cyclophilin A Rabbit Monoclonal antibody M01308 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-13
Question
Is this M01308 anti-Cyclophilin A Rabbit Monoclonal antibody reactive to the isotypes of PPIA?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The immunogen of M01308 anti-Cyclophilin A Rabbit Monoclonal antibody is A synthesized peptide derived from human Cyclophilin A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-31
Question
I see that the anti-Cyclophilin A Rabbit Monoclonal antibody M01308 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-31
Question
Would anti-Cyclophilin A Rabbit Monoclonal antibody M01308 work on horse WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Our lab technicians have not validated anti-Cyclophilin A Rabbit Monoclonal antibody M01308 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Cyclophilin A Rabbit Monoclonal antibody M01308 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-05
Question
Does M01308 anti-Cyclophilin A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Williams
Verified customer
Asked: 2019-09-04
Answer
As indicated on the product datasheet, M01308 anti-Cyclophilin A Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-04
Question
Is a blocking peptide available for product anti-Cyclophilin A Rabbit Monoclonal antibody (M01308)?
E. Dhar
Verified customer
Asked: 2014-08-07
Answer
We do provide the blocking peptide for product anti-Cyclophilin A Rabbit Monoclonal antibody (M01308). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-08-07