Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody

Cyclophilin A antibody

Boster Bio Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody catalog # M01308. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01308
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody

View all Cyclophilin A Antibodies

SKU/Catalog Number

M01308

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody catalog # M01308. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01308)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AABG-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cyclophilin A

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01308 is reactive to PPIA in Human, Mouse, Rat

Applications

M01308 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

51-55 kDa

Calculated molecular weight

18.012kDa

Background of Cyclophilin A

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For PPIA (Source: Uniprot.org, NCBI)

Gene Name

PPIA

Full Name

Peptidyl-prolyl cis-trans isomerase A

Weight

18.012kDa

Superfamily

cyclophilin-type PPIase family

Alternative Names

Cyclophilin A; Cyclosporin A-binding protein; CYPA; CYPAMGC12404; CYPH; EC 5.2.1.8; MGC117158; MGC23397; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase A; Rotamase A; Rotamase; T cell cyclophilin PPIA CYPA, CYPH, HEL-S-69p peptidylprolyl isomerase A peptidyl-prolyl cis-trans isomerase A|PPIase A|T cell cyclophilin|cyclosporin A-binding protein|epididymis secretory sperm binding protein Li 69p|peptidylprolyl isomerase A (cyclophilin A)|rotamase A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPIA, check out the PPIA Infographic

PPIA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Cyclophilin A PPIA Rabbit Monoclonal Antibody

Question

Do you have a BSA free version of anti-Cyclophilin A Rabbit Monoclonal antibody M01308 available?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cyclophilin A Rabbit Monoclonal antibody M01308 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-13

Question

Is this M01308 anti-Cyclophilin A Rabbit Monoclonal antibody reactive to the isotypes of PPIA?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The immunogen of M01308 anti-Cyclophilin A Rabbit Monoclonal antibody is A synthesized peptide derived from human Cyclophilin A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-31

Question

I see that the anti-Cyclophilin A Rabbit Monoclonal antibody M01308 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-31

Question

Would anti-Cyclophilin A Rabbit Monoclonal antibody M01308 work on horse WB with brain?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

Our lab technicians have not validated anti-Cyclophilin A Rabbit Monoclonal antibody M01308 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Cyclophilin A Rabbit Monoclonal antibody M01308 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-05

Question

Does M01308 anti-Cyclophilin A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Williams

Verified customer

Asked: 2019-09-04

Answer

As indicated on the product datasheet, M01308 anti-Cyclophilin A Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-04

Question

Is a blocking peptide available for product anti-Cyclophilin A Rabbit Monoclonal antibody (M01308)?

E. Dhar

Verified customer

Asked: 2014-08-07

Answer

We do provide the blocking peptide for product anti-Cyclophilin A Rabbit Monoclonal antibody (M01308). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-08-07

Order DetailsPrice
M01308

100μL

$370
M01308-30ul

30μL sample

$99
M01308-carrier-free

Carrier Free

$370

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M01308
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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