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Product Info Summary
| SKU: | PA2151 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclophilin B/PPIB Antibody Picoband®
SKU/Catalog Number
PA2151
BA4119-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclophilin B/PPIB Antibody catalog # PA2151. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclophilin B/PPIB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2151)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cyclophilin B, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2151 is reactive to PPIB in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
23.7 kDa
Background of PPIB
Peptidyl-prolyl cis-trans isomerase B, also known as CYPB, is an enzyme that in humans is encoded by the PPIB gene. This gene is mapped to 15q22.31. The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2151 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Positive Control
WB: human U87 whole cell, human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human tonsil tissue, mouse intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B/PPIB antigen affinity purified polyclonal antibody (Catalog # PA2151) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin B/PPIB at approximately 18 kDa. The expected band size for Cyclophilin B/PPIB is at 24 kDa.
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IHC analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151).
Cyclophilin B/PPIB was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cyclophilin B/PPIB Antibody (PA2151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151).
Cyclophilin B/PPIB was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cyclophilin B/PPIB Antibody (PA2151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Cyclophilin B/PPIB Antibody Picoband® (PA2151)
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Customer Q&As
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3 Customer Q&As for Anti-Cyclophilin B/PPIB Antibody Picoband®
Question
Has PA2151 been tested with ICC/IF application on mouse samples?
Verified customer
Asked: 2019-04-23
Answer
The ICC/IF test result on mouse samples is not good. We do not suggest using the Anti-Cyclophilin B/PPIB Antibody (PA2151) for ICC/IF.
Boster Scientific Support
Answered: 2019-05-07
Question
Can PA2151 be used for mouse paraffin-embedded section staining?
Verified customer
Asked: 2019-04-23
Answer
Yes, the Anti-Cyclophilin B/PPIB Antibody (PA2151) can be used for mouse paraffin-embedded section staining.
Boster Scientific Support
Answered: 2019-05-07
Question
We are currently using anti-Cyclophilin B/PPIB antibody PA2151 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
The anti-Cyclophilin B/PPIB antibody (PA2151) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-13
