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Product Info Summary
| SKU: | M02803 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Cyclophilin F Rabbit Monoclonal Antibody
SKU/Catalog Number
M02803
BM5301 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclophilin F Rabbit Monoclonal Antibody catalog # M02803. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclophilin F Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02803)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACGO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclophilin F
Reactive Species
M02803 is reactive to PPIF in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
22.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02803 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-10000
IHC 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human HEL whole cell, human PC-3 whole cell, human HepG2 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse RAW2647 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of Cyclophilin F using anti-Cyclophilin F antibody (M02803).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin F antigen affinity purified monoclonal antibody (Catalog # M02803) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin F at approximately 18 kDa. The expected band size for Cyclophilin F is at 22 kDa.
Specific Publications For Anti-Cyclophilin F Rabbit Monoclonal Antibody (M02803)
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8 Customer Q&As for Anti-Cyclophilin F Rabbit Monoclonal Antibody
Question
I have a question about product M02803, anti-Cyclophilin F Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02803 anti-Cyclophilin F Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-17
Question
you antibody to test anti-Cyclophilin F Monoclonal antibody M02803 on mouse blood ovary for research purposes, then I may be interested in using anti-Cyclophilin F Monoclonal antibody M02803 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The products we sell, including anti-Cyclophilin F Monoclonal antibody M02803, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-28
Question
What sample target on Western Blot application was tested with M02803?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of Cyclophilin F expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
Will anti-Cyclophilin F Monoclonal antibody M02803 work for WB with blood ovary?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of blood ovary, PPIF is highly expressed in blood ovary. So, it is likely that anti-Cyclophilin F Monoclonal antibody M02803 will work for WB with blood ovary.
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-Cyclophilin F Monoclonal antibody M02803 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-Cyclophilin F Monoclonal antibody (M02803) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
I see that the anti-Cyclophilin F Monoclonal antibody M02803 works with WB, what is the protocol used to produce the result images on the product page?
K. Huang
Verified customer
Asked: 2019-04-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-04-01
Question
Is a blocking peptide available for product anti-Cyclophilin F Monoclonal antibody (M02803)?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
We do provide the blocking peptide for product anti-Cyclophilin F Monoclonal antibody (M02803). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-26
Question
Is there a BSA free version of anti-Cyclophilin F Monoclonal antibody M02803 available?
D. Johnson
Verified customer
Asked: 2014-09-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cyclophilin F Monoclonal antibody M02803 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-09-18
