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Product Info Summary
| SKU: | A06562 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CYFIP2 Antibody
SKU/Catalog Number
A06562
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CYFIP2 Antibody catalog # A06562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYFIP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06562)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2B4
Isotype
IgG
Immunogen
Synthesized peptide derived from CYFIP2 at AA range: 1171-1220.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06562 is reactive to CYFIP2 in Human, Mouse, Rat
Applications
A06562 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
148.399kDa
Background of CYFIP2
Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 HeLa cells using CYFIP2 Polyclonal antibody diluted at 1:500.
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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CYFIP2 (Source: Uniprot.org, NCBI)
Gene Name
CYFIP2
Full Name
Cytoplasmic FMR1-interacting protein 2
Weight
148.399kDa
Superfamily
CYFIP family
Alternative Names
Cytoplasmic FMR1-interacting protein 2 CYFIP2 DEE65, EIEE65, PIR121 cytoplasmic FMR1 interacting protein 2 cytoplasmic FMR1-interacting protein 2|p53-inducible protein 121
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYFIP2, check out the CYFIP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYFIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYFIP2 Antibody (A06562)
Hello CJ!
No publications found for A06562
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CYFIP2 Antibody
Question
Will anti-CYFIP2 antibody A06562 work on bovine IHC with brain {eco:0000312?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not tested anti-CYFIP2 antibody A06562 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CYFIP2 antibody A06562 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain {eco:0000312 in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-CYFIP2 antibody A06562 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-CYFIP2 antibody (A06562) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
See attached the WB image, lot number and protocol we used for lymph {eco:0000312 using anti-CYFIP2 antibody A06562. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
I was wanting to use your anti-CYFIP2 antibody for WB for rat lymph eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for rat lymph {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
You can see on the product datasheet, A06562 anti-CYFIP2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lymph {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-23
Question
I see that the anti-CYFIP2 antibody A06562 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-06
