Anti-CYP26A1 Antibody Picoband™

Product Name

Anti-CYP26A1 Antibody Picoband™

View all CYP26A1 Antibodies

SKU/Catalog Number

A03646-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CYP26A1 Antibody Picoband™ catalog # A03646-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CYP26A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03646-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CYP26A1, identical to the related mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03646-3 is reactive to CYP26A1 in Human, Mouse, Rat

Applications

A03646-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

56.199kDa

Background of CYP26A1

Cytochrome P450 26A1 is a protein that in humans is encoded by the CYP26A1 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CYP26A1 using anti-CYP26A1 antibody (A03646-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat H9C2(2-1) whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP26A1 antigen affinity purified polyclonal antibody (Catalog # A03646-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP26A1 at approximately 56 kDa. The expected band size for CYP26A1 is at 56 kDa.

Gene/Protein Information For CYP26A1 (Source: Uniprot.org, NCBI)

Alternative Names

CP26; CYP26Retinoic acid 4-hydroxylase; cytochrome P450 26A1; Cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, family 26, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIA, polypeptide 1; Cytochrome P450RAI; EC 1.14; hP450RAI; P450, retinoic acid-inactivating, 1; P450RAI; P450RAI1Retinoic acid-metabolizing cytochrome CYP26A1 CP26, CYP26, P450RAI, P450RAI1 cytochrome P450 family 26 subfamily A member 1 cytochrome P450 26A1|P450, retinoic acid-inactivating, 1|cytochrome P450 retinoic acid-inactivating 1|cytochrome P450, family 26, subfamily A, polypeptide 1|cytochrome P450, subfamily XXVIA, polypeptide 1|cytochrome P450RAI|hP450RAI|retinoic acid 4-hydroxylase|retinoic acid-metabolizing cytochrome

For more info on CYP26A1, check out the CYP26A1 Infographic

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In this infographic, you will see the following information for CYP26A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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