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Product Info Summary
| SKU: | A00947 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYP2A6V2 Antibody
View all Cytochrome P450 2A6 Antibodies
SKU/Catalog Number
A00947
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CYP2A6V2 Antibody (Catalog # A00947). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP2A6V2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00947)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CYP2A6V2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00947 is reactive to CYP2A6 in Human
Applications
A00947 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56.517kDa
Background of Cytochrome P450 2A6
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
Fernandez-Salguero P., Am. J. Hum. Genet. 57:651-660(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat/HeLa cells, using CYP2A6V2 antibody A00947.
Protein Target Info & Infographic
Gene/Protein Information For CYP2A6 (Source: Uniprot.org, NCBI)
Gene Name
CYP2A6
Full Name
Cytochrome P450 2A6
Weight
56.517kDa
Superfamily
cytochrome P450 family
Alternative Names
Coumarin 7-hydroxylase; CPA6; CYP2A3P450C2A; CYP2AEC 1.14.14.1; CYPIIA6; cytochrome P450 2A6; Cytochrome P450 IIA3; Cytochrome P450(I); cytochrome P450, family 2, subfamily A, polypeptide 6; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6; flavoprotein-linked monooxygenase; P450PB; xenobiotic monooxygenase CYP2A6 CPA6, CYP2A, CYP2A3, CYPIIA6, P450C2A, P450PB cytochrome P450 family 2 subfamily A member 6 cytochrome P450 2A6|1,4-cineole 2-exo-monooxygenase|coumarin 7-hydroxylase|cytochrome P450 IIA3|cytochrome P450(I)|cytochrome P450, family 2, subfamily A, polypeptide 6|cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6|flavoprotein-linked monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2A6, check out the CYP2A6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP2A6V2 Antibody (A00947)
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No publications found for A00947
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CYP2A6V2 Antibody
Question
Would A00947 anti-CYP2A6V2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
It shows on the product datasheet, A00947 anti-CYP2A6V2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-CYP2A6V2 antibody A00947 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-CYP2A6V2 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
It shows on the product datasheet, A00947 anti-CYP2A6V2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-25
Question
Can you help my question with product A00947, anti-CYP2A6V2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Baker
Verified customer
Asked: 2018-04-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00947 anti-CYP2A6V2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-20
