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Product Info Summary
| SKU: | A10081 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-CYS1 Antibody (C-term)
SKU/Catalog Number
A10081
Size
80 µl
Description
Boster Bio Anti-CYS1 Antibody (C-term) (Catalog # A10081). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CYS1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10081)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CYS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide with amino acid positions 128-158 of human CYS1.
Reactive Species
A10081 is reactive to CYS1 in Human, Mouse
Calculated molecular weight
16.4 kDa
Background of CYS1
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10081 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from human heart, human kidney and mouse kidney tissue lysate (from left to right), using CYS1 Antibody (C-term). A10081 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Immunohistochemical analysis of paraffin-embedded H.kidney section using CYS1 Antibody (C-term). A10081 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Specific Publications For Anti-CYS1 Antibody (C-term) (A10081)
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