Anti-Cytochrome P450 3A7 CYP3A7 Antibody

CYP3A7 antibody

Boster Bio Anti-Cytochrome P450 3A7 CYP3A7 Antibody (Catalog # A05417). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05417
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Cytochrome P450 3A7 CYP3A7 Antibody

View all CYP3A7 Antibodies

SKU/Catalog Number

A05417

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Cytochrome P450 3A7 CYP3A7 Antibody (Catalog # A05417). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 3A7 CYP3A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05417)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human Cytochrome P450 3A7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05417 is reactive to CYP3A7 in Human

Applications

A05417 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

57.47kDa

Background of CYP3A7

This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3AP1 pseudogene and is represented by GeneID:100861540.

Komori M., J. Biochem. 105:161-163(1989).
Gellner K., Pharmacogenetics 11:111-121(2001).
Scherer S.W., Science 300:767-772(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For CYP3A7 (Source: Uniprot.org, NCBI)

Gene Name

CYP3A7

Full Name

Cytochrome P450 3A7

Weight

57.47kDa

Superfamily

cytochrome P450 family

Alternative Names

CYPIIIA7; cytochrome P450, family 3, subfamily A, polypeptide 7; Cytochrome P450-HFLA; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; subfamily IIIA, polypeptide 7; xenobiotic monooxygenase CYP3A7 CP37, CYPIIIA7, P-450(HFL33), P-450111A7, P450-HFLA, P450HLp2 cytochrome P450 family 3 subfamily A member 7 cytochrome P450 3A7|aryl hydrocarbon hydroxylase|cytochrome P450, family 3, subfamily A, polypeptide 7|cytochrome P450, subfamily IIIA, polypeptide 7|cytochrome P450-HFLA|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP3A7, check out the CYP3A7 Infographic

CYP3A7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP3A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Cytochrome P450 3A7 CYP3A7 Antibody

Question

Is this A05417 anti-Cytochrome P450 3A7 antibody reactive to the isotypes of CYP3A7?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

The immunogen of A05417 anti-Cytochrome P450 3A7 antibody is Synthesized peptide derived from internal of human Cytochrome P450 3A7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-11

Question

Can you help my question with product A05417, anti-Cytochrome P450 3A7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Huang

Verified customer

Asked: 2019-09-16

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05417 anti-Cytochrome P450 3A7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-16

Question

Does anti-Cytochrome P450 3A7 antibody A05417 work for WB with fetal liver?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

According to the expression profile of fetal liver, CYP3A7 is highly expressed in fetal liver. So, it is likely that anti-Cytochrome P450 3A7 antibody A05417 will work for WB with fetal liver.

Boster Scientific Support

Answered: 2019-07-22

Question

Here is the WB image, lot number and protocol we used for fetal liver using anti-Cytochrome P450 3A7 antibody A05417. Please let me know if you require anything else.

W. Wu

Verified customer

Asked: 2019-05-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-30

Question

Do you have a BSA free version of anti-Cytochrome P450 3A7 antibody A05417 available?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome P450 3A7 antibody A05417 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-27

Question

We are currently using anti-Cytochrome P450 3A7 antibody A05417 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

S. Jones

Verified customer

Asked: 2016-08-02

Answer

The anti-Cytochrome P450 3A7 antibody (A05417) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-02

Order DetailsPrice
A05417-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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