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Product Info Summary
| SKU: | A06791-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 15 KRT15 Antibody
View all Cytokeratin 15 Antibodies
SKU/Catalog Number
A06791-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 15 KRT15 Antibody catalog # A06791-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 15 KRT15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06791-3)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cytokeratin 15
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06791-3 is reactive to KRT15 in Human, Monkey, Mouse, Rat
Applications
A06791-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.212kDa
Background of Cytokeratin 15
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.
Tsz Kan Fung, J. Biol. Chem., Sep 2002; 277: 35140 - 35149.
Michael T. Tetzlaff, Mol. Cell. Biol., Mar 2004; 24: 2487 - 2498.
Cain H. Yam, Mol. Cell. Biol., Jan 1999; 19: 635 - 645.
Michael T. Tetzlaff, PNAS, Mar 2004; 101: 3338 - 3345.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Figure 3. Immunohistochemistry validation of KRT15 using Anti-Cytokeratin 15 KRT15 Antibody (A06791-3).
Immunohistochemical analysis of paraffin-embedded Human breast cancer using Anti-Cytokeratin 15 polyclonal antibody. Antibody was diluted at 1:100 (4°C
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Figure 1. Western blotting validation for Anti-Cytokeratin 15 KRT15 Antibody A06791-3
Western Blot (WB) analysis of specific cells using Anti-Cytokeratin 15 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 2. Western blotting validation for Anti-Cytokeratin 15 KRT15 Antibody A06791-3
Western Blot (WB) analysis of COS7 cells using Anti-Cytokeratin 15 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KRT15 (Source: Uniprot.org, NCBI)
Gene Name
KRT15
Full Name
Keratin, type I cytoskeletal 15
Weight
49.212kDa
Superfamily
intermediate filament family
Alternative Names
CK15; CK-15; cytokeratin 15; Cytokeratin-15; K15keratin-15, basic; K1CO; keratin 15; keratin, type I cytoskeletal 15; Keratin-15; keratin-15, beta; KRTB; type I cytoskeletal 15 KRT15 CK15, K15, K1CO keratin 15 keratin, type I cytoskeletal 15|CK-15|cytokeratin 15|keratin 15, type I|keratin-15, basic|keratin-15, beta|type I cytoskeletal 15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT15, check out the KRT15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 15 KRT15 Antibody (A06791-3)
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No publications found for A06791-3
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