Product Info Summary
SKU: | M02289 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Cow, Goat, Human, Pig, Rat |
Host: | Mouse |
Application: | IHC |
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Product info
Product Name
Anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal Antibody
SKU/Catalog Number
M02289
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal Antibody (Catalog # M02289). Tested in IHC applications. This antibody reacts with Human, Rat, Cow, Goat, Pig.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02289)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: KRT17/778
Isotype
IgG2b, kappa
Immunogen
Recombinant full-length human KRT17 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02289 is reactive to KRT17 in Cow, Goat, Human, Pig, Rat
Applications
M02289 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.106kDa
Background of KRT17
Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified orsimple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glandssuch as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. Additionally, anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Anti-CK17 Mouse Monoclonal Antibody (KRT17/778).
Click image to see more details
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Anti-CK17 Mouse Monoclonal Antibody (KRT17/778).
Protein Target Info & Infographic
Gene/Protein Information For KRT17 (Source: Uniprot.org, NCBI)
Gene Name
KRT17
Full Name
Keratin, type I cytoskeletal 17
Weight
48.106kDa
Superfamily
intermediate filament family
Alternative Names
39.1; CK-17; cytokeratin-17; K17; keratin 17; keratin, type I cytoskeletal 17; keratin-17; PC; PC2; PCHC1 KRT17 39.1, CK-17, K17, PC, PC2, PCHC1 keratin 17 keratin, type I cytoskeletal 17|cytokeratin-17|keratin 17, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT17, check out the KRT17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal Antibody (M02289)
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No publications found for M02289
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal Antibody
Question
Will anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 work on primate IHC with amniotic fluid?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Our lab technicians have not validated anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate amniotic fluid in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
Is this M02289 anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody reactive to the isotypes of KRT17?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The immunogen of M02289 anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody is Recombinant full-length human KRT17 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
you antibody to test anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 on human amniotic fluid for research purposes, then I may be interested in using anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The products we sell, including anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-20
Question
Would M02289 anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
It shows on the product datasheet, M02289 anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-02
Question
Would anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 work for IHC with amniotic fluid?
S. Krishna
Verified customer
Asked: 2016-11-04
Answer
According to the expression profile of amniotic fluid, KRT17 is highly expressed in amniotic fluid. So, it is likely that anti-Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Monoclonal antibody M02289 will work for IHC with amniotic fluid.
Boster Scientific Support
Answered: 2016-11-04