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Product Info Summary
| SKU: | A12904-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Cytokeratin 80/KRT80 Antibody Picoband®
SKU/Catalog Number
A12904-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytokeratin 80/KRT80 Antibody Picoband® catalog # A12904-2. Tested in WB, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cytokeratin 80/KRT80 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12904-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human KRT80 recombinant protein (Position: K217-E452).
Reactive Species
A12904-2 is reactive to KRT80 in Human, Mouse, Rat
Observed Molecular Weight
47 kDa
Calculated molecular weight
50.5 kDa
Background of KRT80
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene's expression profile shows that it encodes a type II epithelial keratin, although structurally the encoded protein is more like a type II hair keratin. This protein is involved in cell differentiation, localizing near desmosomal plaques in earlier stages of differentiation but then dispersing throughout the cytoplasm in terminally differentiating cells. The type II keratins are clustered in a region of chromosome 12q13. Two transcript variants encoding two different fully functional isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12904-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Mouse, Rat |
| Immunoprecipitation | 0.5-2 μg/ml | Human |
| ELISA | 0.1-0.5 μg/ml |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 80/KRT80 using anti-Cytokeratin 80/KRT80 antibody (A12904-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 80/KRT80 antigen affinity purified polyclonal antibody (A12904-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cytokeratin 80/KRT80 at approximately 47 kDa. The expected band size for Cytokeratin 80/KRT80 is at 51 kDa.
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Immunoprecipitating Cytokeratin 80/KRT80 in U2OS whole cell lysate.
Western blot analysis of Cytokeratin 80/KRT80 using anti-Cytokeratin 80/KRT80 antibody (A12904-2).
Lane 1: U2OS whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-Cytokeratin 80/KRT80 antibody in U2OS whole cell lysate,
Lane 3: anti-Cytokeratin 80/KRT80 antibody (2μg) + U2OS whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 80/KRT80 antigen affinity purified polyclonal antibody (A12904-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 80/KRT80 at approximately 47 kDa. The expected band size for Cytokeratin 80/KRT80 is at 51 kDa.
Specific Publications For Anti-Cytokeratin 80/KRT80 Antibody Picoband® (A12904-2)
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