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- Table of Contents
DAD1 antibody (A06054) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
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Anti-DAD1 Antibody
DAD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight
DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-DAD1 Antibody
View all DAD1 Antibodies
View all DAD1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
DAD1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
DAD1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
12.5 kDa
Background of DAD1
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Cite This Product
Anti-DAD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06054)
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Product Promise
We promise Anti-DAD1 Antibody performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A06054
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026