Anti-DAD1 Antibody

Product Name

Anti-DAD1 Antibody

View all DAD1 Antibodies

SKU/Catalog Number

A06054

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DAD1 Antibody (Catalog # A06054). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DAD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06054)

Host

Rabbit

Contents

DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1.

Reactive Species

A06054 is reactive to DAD1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

12.5 kDa

Background of DAD1

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06054 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DAD1

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