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Product Info Summary
| SKU: | A02756-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DAP1/DAP Antibody Picoband®
SKU/Catalog Number
A02756-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DAP1/DAP Antibody Picoband® catalog # A02756-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DAP1/DAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02756-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DAP1/DAP, which shares 92.9% amino acid (aa) sequence identity with both mouse and rat DAP1/DAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02756-3 is reactive to DAP in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
12252 MW
Background of DAP1
Death-associated protein 1 is a protein that in humans is encoded by the DAP gene. This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02756-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human U-937 whole cell, human RT4 whole cell, human PC-3 whole cell, human Hela whole cell, human HepG2 whole cell, human U20S whole cell, human HL-60 whole cell
IHC: human lung cancer tissue
ICC/IF: SiHa cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DAP1/DAP using anti-DAP1/DAP antibody (A02756-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-937 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAP1/DAP antigen affinity purified polyclonal antibody (Catalog # A02756-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAP1/DAP at approximately 15 kDa. The expected band size for DAP1/DAP is at 15 kDa.
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Figure 2. IHC analysis of DAP1/DAP using anti-DAP1/DAP antibody (A02756-3).
DAP1/DAP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAP1/DAP Antibody (A02756-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of DAP1/DAP using anti-DAP1/DAP antibody (A02756-3).
DAP1/DAP was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAP1/DAP Antibody (A02756-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of HL-60 cells using anti-DAP1/DAP antibody (A02756-3).
Overlay histogram showing HL-60 cells stained with A02756-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAP1/DAP Antibody (A02756-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DAP (Source: Uniprot.org, NCBI)
Gene Name
DAP
Full Name
Death-associated protein 1
Weight
12252 MW
Alternative Names
Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; PPBP; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; NAP-2 DAP death associated protein death-associated protein 1|DAP-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAP, check out the DAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAP1/DAP Antibody Picoband® (A02756-3)
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