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Product Info Summary
| SKU: | A03412-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DBC-1/CCAR2 Antibody Picoband™
SKU/Catalog Number
A03412-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DBC-1/CCAR2 Antibody Picoband™ catalog # A03412-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DBC-1/CCAR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03412-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DBC-1/CCAR2 recombinant protein (Position: L65-E917).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03412-1 is reactive to CCAR2 in Human, Monkey, Mouse, Rat
Applications
A03412-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
102.902kDa
Background of DBC1/p30
Deleted in bladder cancer protein 1 is a protein that in humans is encoded by the DBC1 gene. Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A04887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBC-1/CCAR2 antigen affinity purified polyclonal antibody (Catalog # A04887-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBC-1/CCAR2 at approximately 130 kDa. The expected band size for DBC-1/CCAR2 is at 130 kDa.
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Figure 2. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human laryngeal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human pancreatic carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 10. IHC analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 11. IF analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1).
DBC-1/CCAR2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DBC-1/CCAR2 Antibody (A03412-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 12. Flow Cytometry analysis of A431 cells using anti-DBC-1/CCAR2 antibody (A03412-1).
Overlay histogram showing A431 cells stained with A03412-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBC-1/CCAR2 Antibody (A03412-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CCAR2 (Source: Uniprot.org, NCBI)
Gene Name
CCAR2
Full Name
Cell cycle and apoptosis regulator protein 2
Weight
102.902kDa
Alternative Names
DBC.1; DBC1deleted in breast cancer 1; DBC-1p30 DBC protein; Deleted in breast cancer gene 1 protein; KIAA1967; NET35; p30 DBC; p30DBC CCAR2 DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC cell cycle and apoptosis regulator 2 cell cycle and apoptosis regulator protein 2|cell division cycle and apoptosis regulator protein 2|deleted in breast cancer 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCAR2, check out the CCAR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCAR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DBC-1/CCAR2 Antibody Picoband™ (A03412-1)
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