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Product Info Summary
| SKU: | M03089 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DCAMKL1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03089
BM5041 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DCAMKL1 Rabbit Monoclonal Antibody catalog # M03089. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCAMKL1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03089)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAFA-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DCAMKL1
Reactive Species
M03089 is reactive to DCLK1 in Human, Mouse, Rat
Observed Molecular Weight
82 kDa
Calculated molecular weight
82.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03089 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:500
Validation Images & Assay Conditions
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Western blot analysis of DCLK1 using anti-DCLK1 antibody (M03089).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCLK1 antigen affinity purified monoclonal antibody (M03089) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCLK1 at approximately 82 kDa. The expected band size for DCLK1 is at 82 kDa.
Specific Publications For Anti-DCAMKL1 Rabbit Monoclonal Antibody (M03089)
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Customer Q&As
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6 Customer Q&As for Anti-DCAMKL1 Rabbit Monoclonal Antibody
Question
What is the immunogen sequence of M03089?
Verified customer
Asked: 2020-10-14
Answer
The immunogen sequence of the Anti-DCAMKL1 Rabbit Monoclonal Antibody (M03089) is KIKKHFNTGPKPNST aa664-678.
Boster Scientific Support
Answered: 2020-10-14
Question
I was wanting to use your anti-DCAMKL1 Rabbit Monoclonal antibody for IF for mouse fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
It shows on the product datasheet, M03089 anti-DCAMKL1 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
Question
I have a question about product M03089, anti-DCAMKL1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03089 anti-DCAMKL1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-DCAMKL1 Rabbit Monoclonal antibody M03089. Please let me know if you require anything else.
R. Roberts
Verified customer
Asked: 2016-05-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-18
Question
Does anti-DCAMKL1 Rabbit Monoclonal antibody M03089 work on zebrafish WB with hippocampus?
J. Krishna
Verified customer
Asked: 2015-11-05
Answer
Our lab technicians have not tested anti-DCAMKL1 Rabbit Monoclonal antibody M03089 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DCAMKL1 Rabbit Monoclonal antibody M03089 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish hippocampus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-05
Question
We are currently using anti-DCAMKL1 Rabbit Monoclonal antibody M03089 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
W. Moore
Verified customer
Asked: 2014-08-14
Answer
The anti-DCAMKL1 Rabbit Monoclonal antibody (M03089) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-14
