|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-DCC Antibody|
|Description||Rabbit IgG polyclonal antibody for Netrin receptor DCC(DCC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-DCC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1910)|
|Replacement Item||This antibody may replace the following items: sc-6534|sc-6535|sc-11437 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human DCC(162-178aa EVIGEPMPTIHWQKNQQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Netrin receptor DCC|
|Molecular Weight||158457 MW|
|Protein Function||Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. .|
|Tissue Specificity||Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells. .|
|Sequence Similarities||Belongs to the immunoglobulin superfamily. DCC family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Netrin receptor DCC;Colorectal cancer suppressor;Immunoglobulin superfamily DCC subclass member 1;Tumor suppressor protein DCC;DCC;IGDCC1;|
|Research Areas|||cell biology|apoptosis|receptors|associated proteins| neuroscience|neurology process|growth and development|axonal guidance proteins|neurogenesis| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|cell death||
Background for Netrin receptor DCC
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-DCC Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-DCC Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HELA Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,