Anti-DCLK2 Antibody

Product Name

Anti-DCLK2 Antibody

View all DCLK2 Antibodies

SKU/Catalog Number

A09671-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DCLK2 Antibody (Catalog # A09671-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DCLK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DCLK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09671-1)

Host

Rabbit

Contents

DCLK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DCLK2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK2. The immunogen is located within the last 50 amino acids of DCLK2.

Reactive Species

A09671-1 is reactive to DCLK2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.6 kDa

Background of DCLK2

DCLK2 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK2 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09671-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DCLK2 antibody can be used for detection of DCLK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DCLK2

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