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Product Info Summary
| SKU: | A09671 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DCLK2/Dcamkl2 Antibody
SKU/Catalog Number
A09671
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DCLK2/Dcamkl2 Antibody (Catalog # A09671). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCLK2/Dcamkl2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09671)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DCLK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09671 is reactive to Dclk2 in Human, Mouse
Applications
A09671 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
82.979kDa
Background of DCAMKL2
Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm By similarity.
Totoki Y., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 434:724-731(2005).
The German cDNA consortium, Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, using DCLK2 antibody A09671.
Protein Target Info & Infographic
Gene/Protein Information For Dclk2 (Source: Uniprot.org, NCBI)
Gene Name
Dclk2
Full Name
Serine/threonine-protein kinase DCLK2
Weight
82.979kDa
Superfamily
protein kinase superfamily
Alternative Names
CL2EC 2.7.11.1; CLICK-II; CLIK2; DCDC3; DCDC3BCaMK-like CREB regulatory kinase 2; DCK2DCAMKL2; DKFZp761I032; doublecortin and CaM kinase-like 2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; doublecortin-like kinase 2CLICK2; EC 2.7.11; MGC45428; serine/threonine-protein kinase DCLK2 Dclk2|6330415M09Rik, AU044875, CL2, CLICK2, Clic, Click-II, Dcamk, Dcamkl2|doublecortin-like kinase 2|serine/threonine-protein kinase DCLK2|caMK-like CREB regulatory kinase 2|doublecortin and CaM kinase-like 2|doublecortin-like and CAM kinase-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Dclk2, check out the Dclk2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Dclk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DCLK2/Dcamkl2 Antibody (A09671)
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No publications found for A09671
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-DCLK2/Dcamkl2 Antibody
Question
Can you help my question with product A09671, anti-DCLK2/Dcamkl2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09671 anti-DCLK2/Dcamkl2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-13
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-DCLK2/Dcamkl2 antibody A09671. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-20
Question
We are currently using anti-DCLK2/Dcamkl2 antibody A09671 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
M. Anderson
Verified customer
Asked: 2015-02-06
Answer
The anti-DCLK2/Dcamkl2 antibody (A09671) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-06
