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Product Info Summary
| SKU: | M01053 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-DCX/Doublecortin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01053
BM4889 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DCX/Doublecortin Rabbit Monoclonal Antibody catalog # M01053. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCX/Doublecortin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01053)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOAD-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DCX
Reactive Species
M01053 is reactive to DCX in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
40.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01053 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50-1:200
Positive Control
WB: C6 cell
Validation Images & Assay Conditions
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Western blot analysis of DCX expression in C6 cell lysate.
Specific Publications For Anti-DCX/Doublecortin Rabbit Monoclonal Antibody (M01053)
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Customer Q&As
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3 Customer Q&As for Anti-DCX/Doublecortin Rabbit Monoclonal Antibody
Question
We are currently using anti-DCX/Doublecortin Rabbit Monoclonal antibody M01053 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
L. Miller
Verified customer
Asked: 2020-03-02
Answer
The anti-DCX/Doublecortin Rabbit Monoclonal antibody (M01053) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-02
Question
My team were content with the WB result of your anti-DCX/Doublecortin Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma cytoplasm. cell projection, using this antibody. Is that expected? Could you tell me where is DCX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
From what I have seen in literature, cervix carcinoma does express DCX. Generally DCX expresses in cytoplasm. cell projection,. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2019-11-01
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-DCX/Doublecortin Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
From what I have seen in literature cervix carcinoma does express DCX. From what I have seen in Uniprot.org, DCX is expressed in frontal cortex, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2017-06-06
