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Product Info Summary
| SKU: | A02019-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-REDD1/DDIT4 specific Antibody
SKU/Catalog Number
A02019-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-REDD1/DDIT4 specific Antibody catalog # A02019-2. Tested in WB, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-REDD1/DDIT4 specific Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02019-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human REDD1/DDIT4 specific recombinant protein (Position: M1-L228).
Reactive Species
A02019-2 is reactive to DDIT4 in Human, Mouse, Rat
Calculated molecular weight
25.4 kDa
Background of DDIT4
Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02019-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunoprecipitation, 1:50
ELISA, 1:100-1000
Validation Images & Assay Conditions
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Western blot analysis of REDD1/DDIT4 using anti-REDD1/DDIT4 antibody (A02019-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REDD1/DDIT4 antigen affinity purified polyclonal antibody (A02019-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for REDD1/DDIT4 at approximately 35 kDa. The expected band size for REDD1/DDIT4 is at 25 kDa.
Specific Publications For Anti-REDD1/DDIT4 specific Antibody (A02019-2)
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