Click image to see more details
Product Info Summary
| SKU: | M00244 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DDX58 Monoclonal Antibody
SKU/Catalog Number
M00244
BM5264 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DDX58 Monoclonal Antibody catalog # M00244. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX58 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00244)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEA-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DDX58 Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN.
Reactive Species
M00244 is reactive to DDX58 in Human
Observed Molecular Weight
107 kDa
Calculated molecular weight
106.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00244 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: human Jurkat whole cell, human SIHA whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of DDX58 using anti-DDX58 antibody (M00244).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified monoclonal antibody (M00244) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 107 kDa. The expected band size for DDX58 is at 107 kDa.
Specific Publications For Anti-DDX58 Monoclonal Antibody (M00244)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DDX58 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-DDX58 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-DDX58 Monoclonal Antibody
Question
I was wanting to use your anti-DDX58 Monoclonal antibody for WB for human skin testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin testis identification?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
It shows on the product datasheet, M00244 anti-DDX58 Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin testis using anti-DDX58 Monoclonal antibody M00244. Let me know if you need anything else.
K. Anderson
Verified customer
Asked: 2019-10-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-DDX58 Monoclonal antibody M00244 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-16
Question
Will anti-DDX58 Monoclonal antibody M00244 work for WB with skin testis?
G. Walker
Verified customer
Asked: 2017-08-30
Answer
According to the expression profile of skin testis, DDX58 is highly expressed in skin testis. So, it is likely that anti-DDX58 Monoclonal antibody M00244 will work for WB with skin testis.
Boster Scientific Support
Answered: 2017-08-30
Question
We are interested in to test anti-DDX58 Monoclonal antibody M00244 on human skin testis for research purposes, then I may be interested in using anti-DDX58 Monoclonal antibody M00244 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Singh
Verified customer
Asked: 2014-02-26
Answer
The products we sell, including anti-DDX58 Monoclonal antibody M00244, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-26
