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Product Info Summary
| SKU: | A02756-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Death-associated protein 1 DAP Antibody
SKU/Catalog Number
A02756-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Death-associated protein 1 DAP Antibody (Catalog # A02756-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Death-associated protein 1 DAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02756-1)
Host
Rabbit
Contents
DAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP.
Cross-reactivity
DAP antibody is human, mouse and rat reactive.
Reactive Species
A02756-1 is reactive to DAP in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
11.2 kDa
Background of DAP
The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02756-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
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Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.
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Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.
Specific Publications For Anti-Death-associated protein 1 DAP Antibody (A02756-1)
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