Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband™

Product Name

Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband™

View all p120-catenin Antibodies

SKU/Catalog Number

A02333-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband™ catalog # A02333-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02333-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human delta 1 Catenin/CAS/CTNND1 recombinant protein (Position: E31-R875).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02333-4 is reactive to CTNND1 in Human, Mouse, Rat

Applications

A02333-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

108.17kDa

Background of p120-catenin

p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Delta 1 Catenin/CAS/CTNND1 using anti-Delta 1 Catenin/CAS/CTNND1 antibody (A02333-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates,
Lane 9: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Delta 1 Catenin/CAS/CTNND1 antigen affinity purified polyclonal antibody (Catalog # A02333-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Delta 1 Catenin/CAS/CTNND1 at approximately 100-110 kDa. The expected band size for Delta 1 Catenin/CAS/CTNND1 is at 108 kDa.

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Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-Delta 1 Catenin/CAS/CTNND1 antibody (A02333-4).
Overlay histogram showing Caco-2 cells stained with A02333-4 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Delta 1 Catenin/CAS/CTNND1 Antibody (A02333-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CTNND1 (Source: Uniprot.org, NCBI)

Alternative Names

Cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta-1; CTNND; KIAA0384P120CAS; p120 catenin; p120; p120(CAS); p120(CTN); p120cas; p120ctn CTNND1 BCDS2, CAS, CTNND, P120CAS, P120CTN, p120, p120(CAS), p120(CTN) catenin delta 1 catenin delta-1|cadherin-associated Src substrate|catenin (cadherin-associated protein), delta 1|p120 catenin

For more info on CTNND1, check out the CTNND1 Infographic

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In this infographic, you will see the following information for CTNND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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