Click image to see more details
Product Info Summary
| SKU: | A00875-2 |
|---|---|
| Size: | 25uL |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DELTA-4 DLL4 Antibody
SKU/Catalog Number
A00875-2
Size
25uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-DELTA-4 DLL4 Antibody (Catalog # A00875-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-DELTA-4 DLL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Reactive Species
A00875-2 is reactive to DLL4 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
74.6 kDa
Background of DLL4
DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00875-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:25,000
IHC: 10 µg/mL to 20 µg/mL
WB: 1:500 - 1:2,500
Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.
Validation Images & Assay Conditions
Click image to see more details
ELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .
Click image to see more details
Western Blot of Rabbit anti-DELTA-4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate . Lane 2: HUVEK Whole Cell Lysate. Lane 3: Human rDLL4. Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate . Load: 10 µg per lane for Whole Cell Lysates or 50 ng for recombinant protein. Primary antibody: DELTA-4 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for DELTA-4. Other band(s): Not Identified.
Click image to see more details
Boster's Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Click image to see more details
Western Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue . Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
Specific Publications For Anti-DELTA-4 DLL4 Antibody (A00875-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DELTA-4 DLL4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-DELTA-4 DLL4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-DELTA-4 DLL4 Antibody
Question
We have seen staining in rat heart left ventricle. What should we do? Is anti-DELTA-4 antibody supposed to stain heart left ventricle positively?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Based on literature heart left ventricle does express DLL4. Based on Uniprot.org, DLL4 is expressed in heart left ventricle, brain, placenta, among other tissues. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344
Boster Scientific Support
Answered: 2019-06-19
Question
My boss were well pleased with the WB result of your anti-DELTA-4 antibody. However we have observed positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is DLL4 supposed to be expressed?
D. Lewis
Verified customer
Asked: 2018-03-20
Answer
From what I have seen in literature, brain does express DLL4. Generally DLL4 expresses in cell membrane. Regarding which tissues have DLL4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10837024, 11134954
Placenta, Pubmed ID: 17728344
Boster Scientific Support
Answered: 2018-03-20
Question
We are currently using anti-DELTA-4 antibody A00875-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
The anti-DELTA-4 antibody (A00875-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-07
Question
We purchased anti-DELTA-4 antibody for IHC on heart left ventricle in a previous experiment. I am using human, and We are going to use the antibody for WB next. We want examining heart left ventricle as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
H. Collins
Verified customer
Asked: 2015-11-23
Answer
I have checked the website and datasheets of our anti-DELTA-4 antibody and I see that A00875-2 has been validated on human in both IHC and WB. Thus A00875-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-11-23
