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- Table of Contents
Des antibody (M01948-4) performs well in IHC, WB and shows reactivity with Human, Mouse, Rabbit, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Desmin using anti-Desmin antibody (M01948-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat C2C12 whole cell lysates.
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were tDesminsferred to a Nitrocellulose membDesmine at 150mA for 50-90 minutes. Blocked the membDesmine with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membDesmine was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat C2C12 whole cell lysates.
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were tDesminsferred to a Nitrocellulose membDesmine at 150mA for 50-90 minutes. Blocked the membDesmine with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membDesmine was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.
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Anti-Desmin Antibody (Monoclonal, 35D56)
Des antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rabbit, Rat | Mouse | IHC, WB |
Tested Applications
Des antibody was positively detected by WB in:
rat heart tissue, rat skeletal muscle tissue, rat C2C12 whole cell, mouse heart tissue, mouse skeletal muscle tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, mouse, rat, rabbit
Immunohistochemistry (Paraffin-embedded Section)
2-4μg/ml
Human, mouse, rat, rabbit
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Recommend
Rabbit
Recommend
Product Information
Product Name
Anti-Desmin Antibody (Monoclonal, 35D56)
View all DES Antibodies
View all DES Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
35D56
Isotype
Mouse IgG1
Immunogen
Desmin from pig stomach.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
53.5 kDa
Background of Des
Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.
Cite This Product
Anti-Desmin Antibody (Monoclonal, 35D56) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01948-4)
Protocols
Western Blot protocol for Des antibody
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Product Promise
We promise Anti-Desmin Antibody (Monoclonal, 35D56) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01948-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026