|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Desmin Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-Desmin Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9105)|
|Replacement Item||This antibody may replace the following items: sc-130593|sc-134338|sc-14026|sc-162734|sc-23879|sc-271677|sc-34200|sc-34201|sc-365130|sc-52326|sc-58744|sc-58745|sc-65983|sc-70961|sc-7559 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||53536 MW|
|Protein Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm. Cell membrane, sarcolemma .|
|Research Areas|||immunology|innate immunity|complement|classical pathway| immunology|alternative pathway||
Background for Desmin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Desmin Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Desmin Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti Desmin (PB9105) at 0.5ug/ml
WB: Recombinant Human Desmin Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti Desmin (PB9105) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac MuscleTissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HT1080 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPA Whole Cell Lysate at 40ug
Lane 9: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Mouse Cardiac Muscle Tissue
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Mouse Skeletal Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,