Product Info Summary
SKU: | A05604-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DFNA5 Antibody
SKU/Catalog Number
A05604-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DFNA5 Antibody catalog # A05604-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DFNA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05604-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
1E3
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human DFNA5. at AA range: 200-280
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05604-1 is reactive to GSDME in Human, Rat
Applications
A05604-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.555kDa
Background of GSDME
DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Protein Target Info & Infographic
Gene/Protein Information For GSDME (Source: Uniprot.org, NCBI)
Gene Name
GSDME
Full Name
Gasdermin-E
Weight
54.555kDa
Superfamily
gasdermin family
Alternative Names
Gasdermin-E GSDME DFNA5, ICERE-1 gasdermin E gasdermin-E|DFNA5, deafness associated tumor suppressor|inversely correlated with estrogen receptor expression 1|non-syndromic hearing impairment protein 5|nonsyndromic hearing impairment protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSDME, check out the GSDME Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSDME: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DFNA5 Antibody (A05604-1)
Hello CJ!
No publications found for A05604-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DFNA5 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-DFNA5 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-DFNA5 Antibody
Question
Would A05604-1 anti-DFNA5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
As indicated on the product datasheet, A05604-1 anti-DFNA5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-30
Question
Is this A05604-1 anti-DFNA5 antibody reactive to the isotypes of DFNA5?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
The immunogen of A05604-1 anti-DFNA5 antibody is Synthesized peptide derived from the Internal region of human DFNA5. at AA rangle: 200-280. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-26
Question
I see that the anti-DFNA5 antibody A05604-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-20
Question
Is a blocking peptide available for product anti-DFNA5 antibody (A05604-1)?
O. Johnson
Verified customer
Asked: 2016-02-03
Answer
We do provide the blocking peptide for product anti-DFNA5 antibody (A05604-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-03
Question
We are currently using anti-DFNA5 antibody A05604-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on goat tissues as well?
W. Li
Verified customer
Asked: 2013-05-22
Answer
The anti-DFNA5 antibody (A05604-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-22