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Product Info Summary
| SKU: | A14578 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DGAT2L3 AWAT1 Antibody
SKU/Catalog Number
A14578
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DGAT2L3 AWAT1 Antibody catalog # A14578. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGAT2L3 AWAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14578)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human AWAT1. AA range:261-310
Reactive Species
A14578 is reactive to AWAT1 in Human, Mouse
Calculated molecular weight
37.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14578 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using DGAT2L3 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of mouse-kidney KB lysis using DGAT2L3 antibody. Antibody was diluted at 1:1000
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Western blot analysis of lysates from 293 cells, using AWAT1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using AWAT1 antibody.
Specific Publications For Anti-DGAT2L3 AWAT1 Antibody (A14578)
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Customer Q&As
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5 Customer Q&As for Anti-DGAT2L3 AWAT1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammalian vulva using anti-DGAT2L3 antibody A14578. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-DGAT2L3 antibody A14578 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The anti-DGAT2L3 antibody (A14578) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-24
Question
Is this A14578 anti-DGAT2L3 antibody reactive to the isotypes of AWAT1?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The immunogen of A14578 anti-DGAT2L3 antibody is Synthesized peptide derived from the C-terminal region of human DGAT2L3. at AA rangle: 230-310. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
I see that the anti-DGAT2L3 antibody A14578 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-17
Question
My question regarding product A14578, anti-DGAT2L3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14578 anti-DGAT2L3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-30
