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Product Info Summary
| SKU: | A09644S66 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-DGK-Delta (S66) DGKD Antibody
SKU/Catalog Number
A09644S66
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DGK-Delta (S66) DGKD Antibody catalog # A09644S66. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGK-Delta (S66) DGKD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09644S66)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 34-88 of Human DGK-δ.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09644S66 is reactive to DGKD in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
134525 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09644S66 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of DGKD using Anti-DGK-Delta (S66) DGKD Antibody (A09644S66).
Immunohistochemistry (IHC) analyzes of DGK-δ (S66) pAb in paraffin-embedded human Rectum carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-DGK-Delta (S66) DGKD Antibody A09644S66
Western blot (WB) analysis of DGK-δ (S66) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:Hela whole cell lysate(40ug)
Lane3:A2780 whole cell lysate(40ug)
Lane4:PC12 whole cell lysate(40ug)
Lane5:3T3-L1 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DGKD (Source: Uniprot.org, NCBI)
Gene Name
DGKD
Full Name
Diacylglycerol kinase delta
Weight
134525 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
Diacylglycerol kinase delta;DAG kinase delta;2.7.1.107;130 kDa diacylglycerol kinase;Diglyceride kinase delta;DGK-delta;DGKD;KIAA0145; DGKD DGK-delta, DGKdelta, dgkd-2 diacylglycerol kinase delta diacylglycerol kinase delta|DAG kinase delta|diacylglycerol kinase, delta 130kDa|diglyceride kinase delta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGKD, check out the DGKD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGKD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DGK-Delta (S66) DGKD Antibody (A09644S66)
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